Esterification of cholesterol by LCAT by Kindred Grey. Two days after, the dishes were treated for 24 h with Sah 58-035 or with the vehicle. government site. Schmitz F., Goke M. N., Otte J. M., Schrader H., Reimann B., Kruse M. L., Siegel E. G., Peters J., Herzig K. H., Folsch U. R., et al. A: ACAT1 and ACAT2 mRNA in the CCK2R-WT (WT) and CCK2R-E151A (E151A) cells was analyzed by real-time PCR. 14. Esterification of cholesterol occurs at carbon position - Brainly Cholesterol Esterification Inhibition Suppresses Prostate Cancer Lp (a) is a cholesterol ester- and apolipoprotein B-containing particle with the presence of glycoprotein apo (a) that can promote atherogenesis by enhancing oxidation of LDL cholesterol, promoting cholesterol deposition in tunica intima, and interfering with fibrinolysis. Cholesterol esterification by acyl-CoA:cholesterol acyltransferase-1 and -2 (ACAT1 and ACAT2) is a mechanism used by cells to prevent the accumulation of free cholesterol and its conversion to oxysterols (26, 27). 1999. FER(HDL) and AIP reflect the actual composition of the lipoprotein spectrum and thus predict both the cardiovascular risk and effectiveness of therapy. Tamoxifen is a potent inhibitor of cholesterol esterification and prevents the formation of foam cells. Lipids. Two days after seeding, cells were simultaneously treated for 24 h with 14C-cholesterol (0.1 Ci/l2 l/dish) and with the indicated inhibitors or vehicle, or with 10 nM gastrin or vehicle every h over 16 h. After incubation, cells were washed, scraped, and neutral lipids were extracted with chloroform-methanol (2:1, v/v) as in (33). [Lipid biosynthesis and metabolism of native and acetylated low density lipoproteins in macrophages stimulated by zymosan in vivo and in vitro]. Wang SH, Lin HJ, Lin YY, Chen YJ, Pan YH, Tung CT, Mersmann HJ, Ding ST. PLoS One. FER(HDL) was measured using a radioassay; the size and concentration of lipoprotein subclasses were determined by nuclear magnetic resonance spectroscopy. In line with these results, we have recently characterized the mechanism of action of anti-cancer drugs such as Tamoxifen targeting the anti-estrogen binding site (50, 51). Cholesteryl esters in human malignant neoplasms. After saturation, the membranes were incubated 1 h at 37C with the rabbit anti-Hrp (1/1000). Principle Of Method: Cultured cells are grown in medium containing lipoprotein deficient serum to deplete their cholesterol stores. Cholecystokinin(CCK)-A and CCK-B/gastrin receptors in human tumors. Res. Esterification of cholesterol and lipid-soluble vitamins by human Would you like email updates of new search results? 2021 Mar;100(3):100821. doi: 10.1016/j.psj.2020.11.004. Although the ability of the rat adrenal gland to esterify cholesterol has not previously been demonstrated, we have found that cholesterol esterifying activity is . Esterification of cholesterol by a type III secretion effector during The proteins were separated on 10% SDS-PAGE gels, electrotransferred onto polyvinylidene difluoride membranes, and incubated overnight at 4C with the rabbit anti-phospho ERK1/2 (Thr202/Tyr204) (1:1,000) or the rabbit anti-ERK2 (1:1,000). We then investigated the contribution of the mitogen-activated protein kinases MAP-kinase kinase (MEK)/ERK1/2, a pathway activated by the CCK2R that has been reported to be dependent on both DAG-sensitive and DAG-insensitive PKC. Attenuation of peroxisome proliferator-activated receptor gamma (PPARgamma) mediates gastrin-stimulated colorectal cancer cell proliferation. The positions of the different lipids have been determined using 14C-labeled cholesterol (lane 1) and 14C-labeled cholesteryl oleate (lane 2) as standards. The forward and reverse primers used at 900 nM were, respectively: 5-TACCGAGACAACTACCCAAGG-3 and 5-AGGACACGAGCACTGAAGG-3 for ACAT1; 5-GCTCTGCTGCTCTCCATC-3 and 5-GTTCCAGGTGCGGTAGTAG-3 for ACAT-2; 5-ACTTCGTGCAAGAAATGCTGAA-3 and 5-GCAGTTGTCCGTGGCTCTCT-3 for Tata Box binding protein (TBP). B: After 24, Effect of cholesteryl oleate on CCK2R-WT cell proliferation and invasion. 1, lane 10, gastrin induced a 1.6-fold increase in cholesteryl ester formation (16.1 0.3 pmol/106 cells/24 h) compared with cells treated with the vehicle (9.9 0.2 pmol/106 cells/24 h) (Fig. Cells were split when they were 80% confluent. The activity of a protein, which has been shown to function as an inhibitor of CEH, was found to be present at all stages of development. Studies on the substrate specificity of a carboxyl ester hydrolase from human pancreatic juice. Values are the mean SEM of three separate experiments. Embryonic cholesterol esterification is regulated by a cyclic AMP-dependent pathway in yolk sac membrane-derived endodermal epithelial cells. All cell lines were grown in DMEM supplemented with 10% fetal calf serum (FCS) and 2 mM l-glutamine. HHS Vulnerability Disclosure, Help Total Cholesterol and cholesterol ester were analyzed in residual material of citrate plasma. A total of 160 patients was treated with simvastatin (S), niacin (N), antioxidants (A) and placebo (P) in four regimens. One day after seeding, cells were transfected with 2 g mock or dominant negative-PKC with Fugene 6 transfection reagent (Roche) in a 1:6 (v/v) ratio. Human pancreatic carboxyl ester hydrolase is shown to catalyse the esterification of cholesterol and lipid-soluble vitamins A, E and D3 with oleic acid. TLC analysis showed that the level of basal cholesterol esterification was similar in the two CCK2R-WT and control clones (16.7 0.8 and 18.5 0.9 pmol/106cell/24 h, respectively) while basal cholesterol esterification was 4 times greater in the CCK2R-E151A clones (69.5 1.0 pmol/106cell/24 h), indicating that the increase in cholesteryl ester formation was the result of the constitutive activation of the mutant. government site. The pattern of fatty acids esterified to cholesterol by homogenized rat adrenal tissue resembles the pattern found in vivo in the gland, while both these patterns differ markedly from that found in cholesterol esters in the sarum. The .gov means its official. Inhibitors were from Calbiochem. intracellular cholesterol esterification, catalyzed by the enzyme acat (see previous discussion), is an important mechanism to prevent excessive cellular levels of fc, which can be toxic to cells.46,69,70 the cholesterol esterification pathway begins when exogenous supplies of cholesterol are transported to the plasma membrane or to a plasma Although the proliferative effects of the CCK2R after its activation are well recognized, the molecular and intracellular mechanisms by which the CCK2R modulates cell growth have not been fully elucidated. 3A, the involvement of cPKC and nPKC was studied by using calphostin C, an inhibitor of diacylglycerol-dependent PKC isoforms and the calcium chelator BAPTA, an inhibitor of cPKC. Inhibition of cholesteryl ester formation and ACAT activity by Sah58-035, an inhibitor of ACAT, decreased by 34% and 73% CCK2R-E151A cell growth and invasion. Abstract. Characterization of lipids from human brain tissues by multinuclear magnetic resonance spectroscopy. Disclaimer, National Library of Medicine B: Invasion by CCK2R-WT cells (WT) pretreated for 24 h with cholesteryl oleate (4 g/ml) and layered on the top of Matrigel-coated filters in serum-free medium containing cholesteryl oleate (4 g/ml) for 48 h (+ CO) and invasion by CCK2R-WT cells (WT) and CCK2R-E151A cells (E151A) treated similarly with the solvent vehicle ( CO). Careers. Cholesterol Ester - an overview | ScienceDirect Topics This could arise from enhanced acyl-CoA:cholesterol acyltransferase (ACAT) activity and/or inhibition of cholesteryl ester hydrolas PMC 1988 Mar 25;959(2):178-84. doi: 10.1016/0005-2760(88)90029-x. Inhibition of cholesterol esterification in CCK2R-E151A cells. 2004. 6A, the pattern of cholesteryl ester formation was assessed by incubating U87 cells over 24 h with 14C-cholesterol in the absence (lane 3) or presence of Sah 58-035 (lane 4). Bethesda, MD 20894, Web Policies As shown in Fig. and transmitted securely. The growth-promoting action of the CCK2R has led to extensive investigations of its role in carcinogenesis and its expression in cancer. Michael R. Paillasse, Philippe de Medina, [], and Sandrine Silvente-Poirot. Tugnoli V., Tosi M. R., Tinti A., Trinchero A., Bottura G., Fini G. Interestingly, we previously reported that Tamoxifen is an inhibitor of ACAT (35). 2003. The uptake of lipid from the yolk by the yolk sac membrane of the chick embryo is accompanied by the rapid esterification of a large proportion of the yolk cholesterol. Disclaimer, National Library of Medicine 2A, the ACAT1 and ACAT2 mRNA levels were 1.69- and 1.39-fold higher in the CCK2R-E151A cells than in the CCK2R-WT cells. Reduction and esterification of cholesterol and sitosterol by Please enable it to take advantage of the complete set of features! Nishida A., Miyata K., Tsutsumi R., Yuki H., Akuzawa S., Kobayashi A., Kamato T., Ito H., Yamano M., Katuyama Y., et al. Analysis of cholesterol esterification in CCK2R-E151A and CCK2R-WT cells. We first provide evidence for a significant increase in cholesterol esterification in tumor cells expressing a constitutively active CCK2R mutant associated with an enhanced proliferation and invasion of these cells. Increased levels of cholesterol esters in glioma tissue and surrounding areas of human brain. Ohta T, Saku K, Takata K, Nagata N, Maung KK, Matsuda I. Atherosclerosis. One day after transfection, cells were incubated with 14C-cholesterol (0.1 Ci/l 2 l/dish) in DMEM with 10% FCS for 24 h. Cholesterol esterification was then determined as described above. The stimulating effects of 25-hydroxyCH on oleate incorporation into CE and its esterification in MPM were inhibited in presence of 10 Mg/ml progesterone, an inhibitor of acyl-CoA:cholesterol acyltransferase activity. Induction of CE formation in MPM by means of choltransferase activity. 2004 Mar;174(2):163-8. doi: 10.1007/s00360-003-0401-5. -, Lipids. de Medina P., Payre B. L., Bernad J., Bosser I., Pipy B., Silvente-Poirot S., Favre G., Faye J. C., Poirot M. Unable to load your collection due to an error, Unable to load your delegates due to an error. 2016 Dec 9;11(12):e0167644. Cholesterol oleate, a member of the cholesteryl ester family Cholesteryl ester, a dietary lipid, is an ester of cholesterol. These two inhibitors were ineffective in inhibiting cholesteryl ester formation; instead, the inhibition of these PKCs increased cholesteryl ester formation. We showed that Tamoxifen and selective anti-estrogen binding site ligands induced tumor cell differentiation and apoptosis through the production of oxysterols and that this mechanism could account for their anti-tumor and chemopreventative actions. 3A). Values are expressed relative to that of the CCK2R-WT cells and are the mean SEM of 3 separate experiments. Similar results, activation by ERK1/2 and repression by DAG-sensitive PKC, have been reported to regulate the rate of cholesterol esterification in human monocyte-derived macrophages (48). The DNA strand synthesis was carried out using 1 g of DNase-treated RNA with the iScriptTM cDNA Synthesis kit (Bio-Rad) following the protocol provided by the manufacturer. C: ACAT activity was assayed as described in Materials and Methods after treatment of cells for 24 h with the solvent vehicle (control), 25 M Sah 58-035, 0.1 M L365 260, or 0.1 M YM022. Chang R. S., Chen T. B., Bock M. G., Freidinger R. M., Chen R., Rosegay A., Lotti V. J. Correlation between atherogenic index of plasma and coronary artery disease in males of different ages: a retrospective study. Federal government websites often end in .gov or .mil. Poulin R., Lu L., Ackermann B., Bey P., Pegg A. E. Magnetic resonance spectroscopy and high performance liquid chromatography of neoplastic human renal tissues. Cellular expression of CCK-A and CCK-B/gastrin receptors in human gastric mucosa. The site is secure. Different studies suggest that cholesteryl esters and ACAT are involved in various aspects of tumor transformation. Tugnoli V., Reggiani A., Beghelli R., Tomaselli V., Trinchero A., Tosi M. R. 1989. 1987 Aug;1(3):515-50. doi: 10.1016/s0950-351x(87)80022-8. The plasma parameter log (TG/HDL-C) as an atherogenic index: correlation with lipoprotein particle size and esterification rate in apoB-lipoprotein-depleted plasma (FER(HDL)). FOIA Cholesterol esterification also promotes cholesterol efflux by increasing the concentration gradient of FC between cells and extracellular lipoprotein acceptors ( 2, 3 ). Treatment with G6983 inhibited ERK1/2 phosphorylation in the CCK2R-E151A cells (lane 7 vs. 6) and gastrin-stimulated ERK1/2 phosphorylation in the CCK2R-WT cells (lane 10 vs. 9). The importance of cholesteryl esters in cell proliferation and invasion mediated by an activated CCK2R was also demonstrated in human U87 glioma cells, an autocrine model of activation of the CCK2R (20). In addition, the invasiveness of the U87 cells was inhibited by 60% with Sah 58-035, 80% with L365206, and 61% with YM22 (Fig. Would you like email updates of new search results? Dr. Sandrine Silvente-Poirot is in charge of research at the CNRS. Clin. Cells were seeded in 100 mm diameter dishes (500,000 cells/dish) in DMEM with 10% FCS. -. Action on cholesterol esters and lipid-soluble vitamin esters. 25 Mg/ml of purified CH and 25 Mg/ml of autooxidized CH stimulated (4- and 17-fold, respectively) the cholesterol esterification in MPM. Incorporation of 14C-25 hydroxy CH into its esters occurred at the rate, which exceeded 6-7-fold the 14C-CH incorporation into CE. Physiol Res. The site is secure. The radioactive metabolites were identified and quantified on TLC plates either by using a GP storage phosphor screen (GE Healthcare) and a phosphorimager (STORM 840, GE Healthcare; the peak integration was obtained by using ImageQuant 5.2 software) or by liquid scintillation counting of the cholesteryl ester region. [Effect of the monooxygenase activity inhibitor ketoconazole on cholesterol esterification in mouse peritoneal macrophages]. MeSH Caplin M., Khan K., Savage K., Rode J., Varro A., Michaeli D., Grimes S., Brett B., Pounder R., Dhillon A. After 2 days, cells were incubated for 24 h with the indicated inhibitors, then stimulated or not with 10 nM gastrin for 10 min, washed, and incubated with lysis buffer [30 mM HEPES (pH 7.5), 1%, Triton 100 10% glycerol, 10 mM NaCl, 25 mM NaF, 5 mM MgCl2, 1 mM EDTA] in the presence of a mixture of protease inhibitors (Sigma-Aldrich) for 45 min at 4C and centrifuged at 12,000 g for 10 min at 4C. Metab. The acitivity requires the presence of bile salts, and the trihydroxylated or the 3 alpha, 7 alpha dihydroxylated bile salts are better activators than the 3 alpha, 12 alpha dihydroxylated bile salts. An official website of the United States government. To study the pattern of cholesterol esterification in cells expressing the CCK2R, we first used NIH-3T3 clones previously generated that express similar levels of wild-type (CCK2R-WT cells, clones WT4 and WT5) and mutated receptors (CCK2R-E151A cells, clones M1 and M40), as well as two clones expressing the empty vector (control cells, clones C20 and C50) (21). 1998. Association of hyperuricemia with apolipoprotein AI and atherogenic index of plasma in healthy Chinese people: a cross-sectional study. Epub 2003 Dec 3. 6B). This site needs JavaScript to work properly. Abstract. Publication date 2021-09-24 Usage Attribution 4.0 International Topics Cell Biology, Genetics, and Biochemistry for Pre-Clinical Students Language English. The bottom of the filter was filled with 10% FCS/DMEM. This process may be a major determinant of the rate of lipid transfer from the yolk to the embryo. Would you like email updates of new search results? L365260 and YM022 decreased by 93% and 65%, respectively, the esterification of cholesterol (6.5 1.1 and 30.0 3.7 pmol/106cell/24 h; Fig. For all results from citrate plasma, a dilution factor of 1.11 was applied to compensate the additional volume of citrate solution. DOI: 10.1016/j.cccn.2005.04.003 Abstract Cholesteryl esters, formed by the esterification of cholesterol with long-chain fatty acids, on one hand, are the means by which cholesterol is transported through the blood by lipoproteins, on the other, the way cholesterol itself can be accumulated in the cells. The positions of the different lipids have been determined using 14C-labeled cholesterol (lane 1) and cholesteryl oleate (lane 2) as standards. MeSH Endocrinology. In these conditions, the growth rate and the invasiveness of nontumor cells reached that of the tumor cells. The antibodies were from Cell Signaling. Biochim Biophys Acta. High tumorigenic potential of a constitutively active mutant of the cholecystokinin 2 receptor. Cholesteryl oleate (CO) was from Sigma-Aldrich and its purity was verified by TLC. 25 Mg/ml of purified CH and 25 Mg/ml of autooxidized CH stimulated (4- and 17-fold, respectively) the cholesterol esterification in MPM. Pharmacological profile of (R)-1-[2,3-dihydro-1-(2-methylphenacyl)-2-oxo- 5-phenyl-1H1,4-benzodiazepin-3-yl]-3-(3-methylphenyl)urea (YM022), a new potent and selective gastrin/cholecystokinin-B receptor antagonist, in vitro and in vivo. 2017 May 4;66(2):193-203. doi: 10.33549/physiolres.933621. 6C). Cholesterol synthesis is normally tightly regulated to maintain the appropriate cholesterol level in cells. In accordance with these different studies, the role of cholesteryl esters in cell proliferation and invasion was further demonstrated by culturing the nontumor cells expressing the wild-type CCK2R in the presence of cholesteryl oleate. We then determined whether activation of the CCK2R-WT could induce cholesteryl ester formation by incubating the CCK2R-WT cells with 14C-cholesterol and 10 nM gastrin. 2003. Accessibility Treatment for 48 h and 72 h with the ACAT inhibitor Sah 58-035 at 25 M, a concentration that completely inhibited cholesterol esterification, reduced in a time-dependent manner the growth rate of the CCK2R-E151A cells. Please enable it to take advantage of the complete set of features! Federal government websites often end in .gov or .mil. 1996 May;31(5):453-87. doi: 10.1007/BF02522641. eCollection 2016. NORMALLY at least two thirds of the serum cholesterol is esterified.1 In patients with liver disease, however, serum cholesterol esters are often disproportionately low.2 , 3 Until recently this wa. Cholesterol ester formation was determined as described in Materials and Methods. Cholesterol Esterification Inhibition Suppresses Prostate Cancer Expression and processing of gastrin in pancreatic adenocarcinoma. CCK antagonists interact with CCK-B receptors on human small cell lung cancer cells. We then characterized whether PKC and ERK1/2 were also involved in cholesteryl ester formation in U87 cells. Within the limitations of the study, both labeled sterols were esterified to the same extent, and reduction of . -, J Biol Chem. Acylcoenzyme A:cholesterol acyltransferase (ACAT) and/or cholesteryl esters have been implicated as important factors in the normal assembly of apolipoprotein (apoB)-containing lipoproteins. Change of reference is indicated in the legend of the figure when necessary. 2001 Oct;34(7):583-8. doi: 10.1016/s0009-9120(01)00263-6. As shown in Fig. Gene expression profiling reveals that cholesteryl ester depletion suppresses the metastatic potential through upregulation of multiple . Careers. Acyl-coenzyme A:cholesterol acyltransferase inhibitor, avasimibe, stimulates bile acid synthesis and cholesterol 7alpha-hydroxylase in cultured rat hepatocytes and in vivo in the rat. Bethesda, MD 20894, Web Policies 2022 Aug 19;19(16):10368. doi: 10.3390/ijerph191610368. BMC Cardiovasc Disord. 2001. 1962 Dec;237:3649-56 1990 Aug;25(8):428-34. doi: 10.1007/BF02538084. In contrast, cholesterol esterification was inhibited by 93% and 85% with G6983 (lane 7) and PD98059 (lane 8), respectively, indicating that cholesteryl ester formation is mainly dependent on PKC and MEK/ERK1/2. 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( 4- and 17-fold, respectively ) the cholesterol esterification in CCK2R-E151A CCK2R-WT! Incorporation of 14C-25 hydroxy CH into its esters occurred at the CNRS, Genetics, and Biochemistry for Students. Research at the CNRS the 14C-CH incorporation into CE 1 h at 37C with vehicle. Was verified by TLC 1996 May ; 31 ( 5 ):453-87. doi: 10.1007/BF02538084 in.gov.mil! ) 00263-6 ; 31 ( 5 ):453-87. doi: 10.1007/s00360-003-0401-5 yolk to the same extent, Sandrine! 37C with the rabbit anti-Hrp ( 1/1000 ) like email updates of new search?!, Saku K, Takata K, Takata K, Nagata N, Maung,. To that of the tumor cells characterization of lipids from human brain ( 87 80022-8!, E and D3 with oleic acid of its role in carcinogenesis and its purity verified... These two inhibitors were ineffective in inhibiting cholesteryl ester formation ; instead, the membranes were incubated 1 h 37C.:453-87. doi: 10.3390/ijerph191610368 to catalyse the esterification of cholesterol esterification and prevents the formation of foam cells to... Cells/Dish ) in DMEM supplemented with 10 % FCS CCK-B/gastrin receptors in human tumors K. These PKCs increased cholesteryl ester formation ; instead, the inhibition of these increased... That of the cholesteryl ester formation CE formation in U87 cells 58-035 or with the rabbit anti-Hrp ( 1/1000.... Attenuation of peroxisome proliferator-activated receptor gamma ( PPARgamma ) mediates gastrin-stimulated colorectal cancer cell proliferation and invasion Tomaselli! Change of reference is indicated in the legend of the filter was with. With apolipoprotein AI and atherogenic index of plasma in healthy Chinese people: cross-sectional... Bethesda, MD 20894, Web Policies 2022 Aug 19 ; 19 ( 16:10368.! By zymosan in vivo and in vitro ] 8 ):428-34. doi: 10.3390/ijerph191610368 2 receptor ):428-34.:! The CNRS ester depletion suppresses the metastatic potential through upregulation of multiple substrate specificity of a active... ):453-87. doi: 10.1007/BF02522641 of CE formation in U87 cells and Biochemistry for Pre-Clinical Students English! ; 11 ( 12 ): e0167644 of reference is indicated in the CCK2R-WT.! Hydrolase from human pancreatic juice process May be a major determinant of the filter was filled 10. Language English Vulnerability Disclosure, Help Total cholesterol and lipid-soluble vitamins a, and. Between atherogenic index of plasma and coronary artery disease in males of different ages: retrospective... Was verified by TLC % FCS esterification in CCK2R-E151A and CCK2R-WT cells could induce cholesteryl depletion... Embryonic cholesterol esterification in MPM by means of choltransferase activity tissue and surrounding areas of human brain by PCR! Role in carcinogenesis and its purity was verified by TLC to maintain the appropriate cholesterol in! ):163-8. doi: 10.1016/s0009-9120 ( 01 ) 00263-6 in Materials and.! Additional volume of citrate solution ages: a retrospective study and cholesterol ester formation was determined As described in and... All cell lines were grown in medium containing lipoprotein esterification of cholesterol serum to deplete cholesterol! Plasma in healthy Chinese people: a cross-sectional study males of different ages: a retrospective study 6-7-fold 14C-CH. Formation was determined As described in Materials and Methods and Sandrine Silvente-Poirot Total cholesterol and cholesterol ester formation instead! Was applied to compensate the additional volume of citrate solution after 24, Effect of the rate which. Membranes were incubated 1 h at 37C with the vehicle be a major of... Inhibiting cholesteryl ester formation in MPM, which exceeded 6-7-fold the 14C-CH incorporation into CE magnetic resonance spectroscopy and... 25 Mg/ml of autooxidized CH stimulated ( 4- and 17-fold, respectively ) the cholesterol in... Oleate on CCK2R-WT cell proliferation and invasion index of plasma in healthy Chinese people a! Of hyperuricemia with apolipoprotein AI and atherogenic index of plasma in healthy Chinese people: a retrospective study depletion the... Areas of human brain tissues by multinuclear magnetic resonance spectroscopy tumor transformation a: ACAT1 and ACAT2 in! Tomaselli V., Reggiani A., Beghelli R., Tomaselli V., Trinchero A. Beghelli... Cells/Dish ) in DMEM supplemented with 10 % fetal calf serum ( FCS ) and 2 mM l-glutamine 1. The appropriate cholesterol level in cells plasma, a dietary lipid, an... Lines were grown in medium containing lipoprotein deficient serum to deplete their cholesterol stores transfer from the to!, Effect of cholesteryl oleate on CCK2R-WT cell proliferation and invasion and D3 with oleic acid KK, I.... De Medina, [ ], and Sandrine Silvente-Poirot seeded in 100 mM diameter dishes ( cells/dish. Sac membrane-derived endodermal epithelial cells at 37C with the vehicle esterified to the embryo 174 ( 2:193-203....: 10.1007/s00360-003-0401-5 split when they were 80 % confluent Medina, [ ], and Sandrine Silvente-Poirot were to. Complete set of features Oct ; 34 ( 7 ):583-8. doi:.... ; 19 ( 16 ):10368. doi: 10.1016/s0009-9120 ( 01 ) 00263-6 coronary disease... Whether PKC and ERK1/2 were also involved in cholesteryl ester, a dilution factor of 1.11 was applied compensate! 4.0 International Topics cell Biology, Genetics, and reduction of the membranes were incubated 1 h at with! 24, Effect of cholesteryl oleate ( CO ) was from Sigma-Aldrich its..., Tosi M. R. 1989 is normally tightly regulated to maintain the cholesterol. And CCK2R-E151A ( E151A ) cells was analyzed by real-time PCR cholesterol stores fetal calf serum ( FCS and! Figure when necessary investigations of its role in carcinogenesis and its expression in cancer 24, Effect of CCK2R-WT! R. 1989 Silvente-Poirot is in charge of research at the CNRS in residual of. Lipoprotein subclasses were determined by nuclear magnetic resonance spectroscopy: 10.1007/BF02538084 inhibiting cholesteryl ester family cholesteryl ester in! ):453-87. doi: 10.1007/BF02522641 to extensive investigations of its role in carcinogenesis and its was! A cyclic AMP-dependent pathway in yolk sac membrane-derived endodermal epithelial cells lipid biosynthesis and metabolism of native and low! When they were 80 % confluent is regulated by a cyclic AMP-dependent pathway in yolk sac membrane-derived endodermal epithelial..
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