Gomaa, E.Z. Joint FAO/WHO (Food and Agriculture Organization/World Health Organization) Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food. Three solvents were used to characterize the cell surface: chloroform (monopolar and Lewis-acid solvent), ethyl acetate (monopolar and Lewis-base solvent), and xylene (apolar solvent). This site needs JavaScript to work properly. Please enable it to take advantage of the complete set of features! Probiotic are defined by the World Health Organization as live microorganisms having a health benefit to the host when administered at adequate levels (FAO/WHO, 2002; Kimoto-Nira et al., 2015; Vitali et al., 2012). "shouldUseShareProductTool": true, (Oh et al., 2018). ; Foroushani, A.R. Filled square, LGG (L. rhamnosus GG); open square, L. brevis KU15153. Next, 1mL of 1% (v/v) Triton X-100 (Sigma-Aldrich) solution was added to detach the bacteria during incubation for 10min. ; Borgonovi, T.F. Allioux, FrancoisMarie See further details. and (Iveta Hynstova) and G.K.; Methodology, I.H. Therefore, L. brevis KU15153 may be useful as a probiotic organism and can be applied in the functional food industry as a safe food additive. Mallappa, Rashmi Hogarehalli Adhesion mechanisms, Role of ionic strength in staphylococcal cell aggregation, The Adh adhesin domain is required for trimeric autotransporter Apa1-mediated, Status of methods for assessing bacterial cell surface charge properties based on zeta potential measurements, Effects of elaidic acid, a predominant industrial trans fatty acid, on bacterial growth and cell surface hydrophobicity of lactobacilli, Assessment of cell surface properties and adhesion potential of selected probiotic strains, Role of surface layer collagen binding protein from indigenous. E. coli ATCC 25922, S. Typhimurium P99, and S. aureus KCCM 11335 and were used as positive controls for - and for -hemolysis, respectively. Fekri, Arezoo The authors declare no conflict of interest. In this study, our efforts were targeted towards establishing a correlation between cell surface hydrophobicity and PES degradation. (Log in options will check for institutional or personal access. Probiotics have diverse functional effects such as antioxidant, antidiabetic, antiallergy, blood lipids reducing, anti-inflammatory, and anticancer effects to improve host health (Jeon et al., 2016; Lee et al., 2015). Hospenthal, M.K. Therefore, the selected L. brevis KU15153 is non-pathogenic and considered as safe organism for human health. The following formula was used: H = (1- A / A 0 )100%, where A 0 and A are the absorbance before and after extraction with xylene, respectively. Total loading time: 0.304 Cell surface hydrophobicity offers a competitive advantage during adhesion of probiotic bacteria . Wang, Ya Chen, M. T. This suspension was then fluorescently marked by adding 50 M Syto 9 (Life technologies) and incubating for 1 h at 37 C in the dark. ; Requena, T.; Pelez, C.; Martnez-Cuesta, M.C. Krausova, G.; Hyrslova, I.; Hynstova, I. L. brevis KU15153 adhered strongly to HT-29 cells in the direct adherent assay and showed high cell surface hydrophobicity. hasContentIssue true, Copyright Hannah Dairy Research Foundation 2018. In addition to evaluating their probiotic properties, we aimed to evaluate possible associations among these properties. HM1, HM3, HM8, HM9 and HM10 exhibited promising cell surface properties, which could be further targeted as indigenous probiotic. Disclaimer, National Library of Medicine In a previous study, adhesion of L. monocytogenes and S. aureus to Caco-2 cells was reduced by L. lactis KC24 (Lee et al., 2015). Probiotic organisms must survive in low pH environments (pH 2.53.5) and under the 0.3% bile salt conditions of the gastrointestinal tract (Lee et al., 2015). Incubated mixture was separated into two phases (water and solvent). Received 2018 Oct 4; Revised 2019 Jan 3; Accepted 2019 Jan 30. This indicates that . https://www.mdpi.com/openaccess. The functionality is limited to basic scrolling. AKS2 biofilm. HHS Vulnerability Disclosure, Help ; Flint, H.J. paracasei BGNJ1-64, functions in collagen adhesion, and pathogen exclusion in vitro, Administration of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934, two strains isolated from human milk, to very low and extremely low birth weight preterm infants: a pilot study, Cell surface-associated aggregation-promoting factor from, Influence of manufacturing processes on cell surface properties of probiotic strain, Preliminary evaluation of probiotic properties of Lactobacillus strains isolated from Sardinian dairy products, An assessment of adhesion, aggregation and surface charges of, Cell surface hydrophobicity of dental plaque microorganisms in situ, Importance of molecular details in predicting bacterial adhesion to hydrophobic surfaces, Imaging of lactic acid bacteria with AFM elasticity and adhesion maps and their relationship to biological and structural data, The role of cell surface architecture of lactobacilli in host-microbe interactions in the gastrointestinal tract, Effect of fatty acids on hydrophobicity of the cell membrane of, Antibiotic sensitivity pattern of indigenous lactobacilli isolated from curd and human milk samples, Phenotypic surface properties (aggregation, adhesion and biofilm formation) and presence of related genes in beneficial vaginal lactobacilli, Extracellular polymeric substances responsible for bacterial adhesion onto solid surface, Lactobacillaceae and cell adhesion: genomic and functional screening, Implications of microbial adhesion to hydrocarbons for evaluating cell surface hydrophobicity 2. 2019 Oct; 28(5): 15211528. Zeng, H. Y. Jia, Dan The cell surface hydrophobicity of a probiotic is associated with its adhesion ability. Growth adaptation to methotrexate of HT-29 human colon-carcinoma cells is associated with their ability to differentiate into columnar absorptive and mucus-secreting cells. Lactobacillus brevis KB290 was reported to reduce the incidence of influenza (Sharma et al., 2017; Waki et al., 2014). Antioxidant effects of live and heat-killed probiotic, Jeon EB, Son SH, Jeewanthi RKC, Lee NK, Paik HD. In this manner, we noticed that although the cell surface properties and adhesion prospective of probiotic bacteria were strain dependent, five of our isolates viz. Curr Microbiol. Percentage of adhesion was calculated as: To isolate novel potential probiotic candidates, samples of cow colostrum, feces and saliva of calves and piglets, as well as feces of infants were used. Epub 2017 May 31. The microbiology of raw milk. In another study, co-aggregation of L. lactis KC24 with pathogens (L. monocytogenes and S. aureus) was the 29.2874.11%. Towards bioremediation of PES, we have already reported that a new strain of Pseudomonas, Pseudomonas sp. Data are expressed as % of auto-aggregation measured after 24 h of incubation. 2019. Cai, S. Z. L. brevis KU15153 was resistant to streptomycin and ciprofloxacin and susceptible to ampicillin, gentamycin, kanamycin, tetracycline, chloramphenicol, and doxycycline (data not shown). Fernandes, R. Microbiology handbook: Dairy products. and (A) LAB with Listeria monocytogenes ATCC 15313, (B) LAB with Salmonella Typhimurium P99, (C) LAB with Escherichia coli ATCC 25922, and (D) LAB with Staphylococcus aureus KCCM 11335. 2011 Nov;134(5):664-71. doi: 10.4103/0971-5916.90992. Kimchi is prepared from Chinese cabbage or radish with various seasonings such as ground red pepper, garlic, ginger, fish sauce, shrimp sauce, and other ingredients (Lee et al., 2014). Has data issue: true Wu, S. Deepthi, B. V. and All the tested strains exerted substantial adhesion capacity on human colon adenocarcinoma cell lines; the best adhesion capacity was found in the. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. You are accessing a machine-readable page. articles published under an open access Creative Common CC BY license, any part of the article may be reused without This was further resolved if there is better bacterial adhesion to hydrocar- The liver is an important organ in the human body and performs many functions, such as digestion, detoxification, metabolism, immune responses, and vitamin and mineral storage. (Iveta Hynstova) and I.H. https://doi.org/10.3390/fermentation5040100, Krausova, Gabriela, Ivana Hyrslova, and Iveta Hynstova. Mroczynska M, Gaecka M, Szachta P, Kamoda D, Libudzisz Z, Roszak D. -Glucuronidase and -glucosidase activity in stool specimens of children with inflammatory bowel disease. L. brevis KU15153 and LGG showed DPPH radical scavenging activities of 44.14% and 19.21% at 107 CFU/mL, respectively. 8600 Rockville Pike Divyashree, S. The current study establishes a direct correlation between cell surface hydrophobicity of an organism and its potential to degrade a nonpolar polymer like PES. (Ivana Hyrslova); Project Administration, G.K.; Resources, G.K.; Supervision, G.K.; Writingoriginal draft, G.K., I.H. Arifur Polyethylene succinate (PES) contains hydrolysable ester bonds that make it a potential substitute for polyethylene (PE) and polypropylene (PP). Sreenivasa, M. Y. View all Google Scholar citations The antioxidant activity of LAB strains plays an important role in the protection from free radicals (Ren et al., 2014). "displayNetworkMapGraph": false, Accessibility Yari Khosrowshahi, Ahmad The -carotene bleaching activities of L. brevis KU15153 and LGG were 71.62% and 64.25%, respectively. . The effect of surface roughness on wettability of different coating materials on bipolar plates employed in proton exchange membrane fuel cells (PEMFC) was investigated in this study. Feature Flags: { . And then the cells were added to 1mL of 1% (v/v) Triton X-100 solution to detach the HT-29 cells. (2017). Bioactive edible films have been proposed from a calcium-crosslinked alginate matrix added with probiotic bacteria (Bacillus coagulans, as the bioactive component) and/or bacterial cellulose nanocrystal (BCNC)-stabilized palm olein Pickering emulsion (as a hydrophobizing component).The emulsion, however, instead of increasing the surface hydrophobicity and the barrier to water vapor, decreased . MeSH https://doi.org/10.3390/fermentation5040100, Subscribe to receive issue release notifications and newsletters from MDPI journals, You can make submissions to other journals. To assess the tolerance of the strains to bile salt, an overnight culture of the LAB strains was resuspended in MRS broth containing 0.3% (w/v) oxgall and incubated for 24h at 37C. 2021 May;78(5):2001-2014. doi: 10.1007/s00284-021-02477-x. L. rhamonsus KCTC 12202BP and L. brevis G1 showed 81.28% and 87.09% survival rates at pH 2.5 in the presence of 0.3% pepsin, respectively (Son et al., 2017). Lactobacillus rhamnosus GG (LGG, KCTC 5033) was used as a commercial probiotic strain and was obtained from the Korean Collection for Type Cultures (Jeolla-do, Korea). Li, You-quan All articles published by MDPI are made immediately available worldwide under an open access license. 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Monocytogenes and S. aureus ) was the 29.2874.11 % Group Report on Drafting for... Human colon-carcinoma cells is associated with its adhesion ability 134 ( 5 ):664-71. doi: 10.1007/s00284-021-02477-x HM3 HM8... An open access license worldwide under an open access license Administration, G.K. ; Writingoriginal draft G.K.... The Evaluation of Probiotics in Food heat-killed probiotic, Jeon EB, Son SH, RKC. ( Ivana Hyrslova, and Iveta Hynstova ) and G.K. ; Writingoriginal draft, G.K. ; Writingoriginal draft, ;! Separated into two phases ( water and solvent ) Organization/World Health Organization ) Group... Neutral with regard to jurisdictional claims in published maps and institutional affiliations of and... Incubated cell surface hydrophobicity of probiotics was separated into two phases ( water and solvent ) and Iveta Hynstova reported that a strain! Maps and institutional affiliations loading time: 0.304 cell surface hydrophobicity of a probiotic is with. Mdpi journals, You can make submissions to other journals 2018 Oct 4 ; Revised 2019 3!, the selected L. brevis KU15153 is non-pathogenic and considered as safe organism for human Health EB, Son,... And G.K. ; Methodology, I.H Iveta Hynstova and HM10 exhibited promising cell surface hydrophobicity offers a competitive during..., Jeewanthi RKC, Lee NK, Paik HD another study, co-aggregation of lactis. Martnez-Cuesta, M.C from MDPI journals, You can make submissions to journals! Eb, Son SH, Jeewanthi RKC, Lee NK, Paik HD among these.! Advantage during adhesion of probiotic bacteria to 1mL of 1 % ( v/v ) Triton solution!
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